2xTaq PCR Mastermix


SKU: N/A Category:

Storage: 2-8℃


  • This product contains phenol red, which may change color from orange to purple. Color change does not affect product quality or use.

Product Description:

  • This product contains Taq DNA polymerase, dNTPs, MgCl2, and reaction buffer at a concentration of 2×. It has the advantages of being fast, easy, sensitive, specific, stable, and can minimize human errors. Only DNA template and primers are needed for use.
  • This product is convenient and easy to use, and can avoid contamination during PCR operation. It only needs to add an appropriate amount of 2×Taq PCR MasterMix solution, add the template and primers, and add deionized water to make the MasterMix solution concentration 1× for the reaction. Please ensure that it is fully dissolved and mixed before use, and the operation should be carried out on ice.

Product Contents:

  • Taq DNA polymerase (recombinant): 0.1 units/µl; MgCl2: 4 mM; dNTPs (dATP, dCTP, dGTP, dTTP): 0.4 mM

Quality Control:

  • No exogenous nuclease activity was detected; it can effectively amplify single-copy genes in the human gene; no significant activity change was observed after one week of storage at room temperature.

Scope of Application:

  • Gene detection: This product has small differences between batches, especially suitable for large-scale gene detection, semi-quantitative PCR experiments, and the detection of trace DNA.
  • Amplification of DNA and some complex templates with special structures and high GC content (>60%) and secondary structures: amplification of DNA fragments, DNA labeling, primer extension, and sequence determination. PCR products are A-tailed and can be directly cloned into T/A vectors after purification.

If you have any questions, please feel free to let us know: market@changyubio.com, thanks.



Data Sheet

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