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2 Weeks Mouse Polyclonal Antibody Quick Adjuvant

Original price was: $249.00.Current price is: $199.00.

2 weeks mouse polyclonal antibody quick adjuvant

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Intended use: standard generation of murine monoclonal and polyclonal antibodies (ELISA titers up to 1:10000~1:1000000) by two immunizations within 5 weeks.

Immunization Protocol

1. Dilute the antigen with saline to 2-fold final concentration (50μl per injection).

Note: Recommended antigen dosage is: (1) 5~20μg per injection for low immunogenic antigens such as recombinant subunit proteins or 20~50μg for peptide-carrier conjugates; (2) 1~5μg per injection for high immunogenic  antigens such as inactivated viruses or recombinant virus-like particles.

2. Mix the adjuvant by vortexing, sterilely remove the required volume (50μl per injection), and mix with the antigen at 1:1 ratio (v/v) as quick as possible.

Note: It is normal to see precipitation in the adjuvant.

3. Inject 100μl of the antigen/adjuvant mixture into a quadriceps muscle of each mouse.

Note: It is normal to see precipitation in the antigen/adjuvant mixture. Please mix well before drawing into syringe and inject as quick as possible after drawing into syringe.

4. Boost animals on day 21 post-priming.

Note: For boosting, please follow steps 1-3 of the priming protocol, using freshly prepared antigen/adjuvant mixture.

5. Bleed from tail tips on day 35 post-priming and measure the antibody titers by ELISA. ELISA titers should be in the range of 1:10000~1:1000000. After that the whole serum can be collected or proceed to antigen boost and cell fusion according to conventional hybridoma protocols.

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size

1ml

Data Sheet

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